Session Details

Experimental Rigor in Phenotyping and Functional Testing by Flow Cytometry (Industry Luncheon Hosted By Beckman Coulter)
Wednesday, May 29, 2019 11:45 AM - 01:00 PM
Room 220

Michael Kapinsky, PhD, Beckman Coulter Life Sciences

Flow cytometry is a core method in characterization of primary human cells as the technology can provide a direct correlation between cellular identity, maturation, activation status, antigen specificity and an observed immune response. Variability of flow cytometry results may impede the method’s value, which can be mitigated through diligent validation, training and standardization, as demonstrated by The ONE Study.

To advance standardization in clinical research beyond The ONE Study gold standard, the concept of scientific rigor, as recently introduced in the NIH grant policies, may be applied that – among other aspects – enforces a robust, unbiased and replicable experimental design. Elimination of sources of human error and variability is recognized as being superior over mitigation strategies. Dry reagent formulation of technically optimized flow cytometry panels can support this goal very efficiently through exclusion of manual pipetting errors and built-in antibody panel accuracy, irrespective of time point, laboratory and operator. Further enhancement can be achieved through integration of dry reagent technology with automation, data management systems and robust flow cytometry instrumentation.