Session Details

Plenary 2 Satellite Session – Enabling Technologies in Regenerative Medicine
Thursday, May 30, 2019 11:00 AM - 12:15 PM 
Room 220
Chair: Tracy Grikscheit (US)

Cell Fiber Technology for 3D Tissue Construction and Cell Therapy
Shoji Takeuchi (JP)

Learning objectives, to understand:
• The cell fiber technology and its advantages
• How to form the cell fiber
• The application of the cell fiber in 3D cell culture and cell therapy

Engineering the Extracellular Microenvironment to Modulate the Therapeutic Properties of Mesenchymal Stromal Cells
Jessica Frith (AU)

Mesenchymal stem/stromal cells (MSCs) show great promise for a wide range of applications including treating graft-vs-host disease, rheumatoid arthritis and cardiovascular diseases, as well as in the tissue-engineering of bone and cartilage. Typical strategies for MSC-based tissue regeneration require significant expansion of the cells in vitro, most commonly using standard tissue culture flasks. However, it is increasingly understood that MSCs are highly sensitive to their extracellular environment and that physical cues from the surrounding microenvironment have a huge influence on the properties of the cells. A thorough understanding of these changes and the mechanisms that drive them therefore has the potential to underpin better ways to both expand and deliver MSCs for therapeutic applications.
We have used biomaterial systems to investigate the effects of different physical cues on the properties of MSCs and understand how mechanotransductive signalling mediates these changes. Our work has shown how miRNA signalling changes in response to the stiffness of the culture substrate and demonstrated that an understanding of these changes can be harnessed to control cell fate. We have also investigated the effects of substrate stiffness on MSC paracrine signalling, showing changes that affect the activity of the MSCs themselves as well as other cell populations, such as macrophages and T-cells, that mediate some of the immunomodulatory effects of MSCs. Investigating a light-curable PEG-gelatin hydrogel for encapsulation and delivery of MSCs, we also highlight the significant differences in cell behaviour that can occur in response to culture format, showing changes in cell migration and differentiation when MSCs are encapsulated in microgels compared to bulk hydrogels.  Together these studies provide insights into the role of the extracellular environment in regulating MSC properties and provide examples of how an understanding of these processes can be applied for cell expansion and delivery.

Selected Oral Abstract Presentation:

Lucy Luo (CA) – Generating Biomimetic Mineralized Collagen Scaffolds for Bone Regeneration (Abstract 11)

Tracy Grikscheit MD
Professor of Surgery
Children's Hospital Los Angeles

Lucy Luo BHSc
MASc Candidate
University of Toronto

Shoji Takeuchi PhD
The University of Tokyo

Jessica Frith PhD
Senior Lecturer
Monash University